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Exoglycosidase digestions of 2-AB labeled human plasma transferrin (Tf) N-glycans. (i) Undigested samples. (ii) Streptococcus pneumoniae α2–3 neuraminidase S. (iii) Arthrobacter ureafaciens α2-3,6,8,9 neuraminidase A (SIA). (iv) Streptococcus pneumoniae β1–4 galactosidase S (GAL) + SIA. (v) Streptococcus pneumoniae β-N-acetylglucosaminidase S (GlcNAc) + GAL + SIA. (vi) Omnitrophica bacterium α1-2,4,6 fucosidase O (FUC) + GlcNAc + Gal + SIA.
