TABLE 1.
Tumor and uterine lavage mutation testing
Tumor testing | Uterine lavage testing | |||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|---|
Patient ID | Ovarian cancer type | Histology | Age at surgery | Stage | Tumor testing platform | Mutation | Protein variant | Coding variant | Tumor mutation found | VAF | Mutant duplex reads | Duplex depth at position |
P15 | Non-serous | Clear-cell carcinoma | 53 | IC3 | DS | ARID1A | p.W1023Vfs*10 | c.3066dup | yes | 0.0134 | 14 | 1046 |
P16 | Non-serous | Clear-cell carcinoma | 64 | IIB | DS | TP53 | P.E221* | c.661G>T | no | |||
P17 | Non-serous | Clear-cell carcinoma | 69 | IA | DS | ARID1A | p.K1808Nfs*4 | c.5423 5424insTTAC | no | |||
P18 | Non-serous | Endometrioid carcinoma | 57 | IC1 | DS | CTNNB1 | P.S33C | c.98C>G | no | |||
P19a | Non-serous | Endometrioid carcinoma | 58 | IC3 | DS | ARID1A CTNNB1 PIK3CA |
p.R1335* p.D32Y P.H1047R |
C.40030T c.94G>T c.3140A>G |
no | |||
P20 | Non-serous | Endometrioid carcinoma | 83 | IA | DS | PIK3CA | P.N1044Y | c.3130A>T | yes | 0.0053 | 16 | 2997 |
P21 | Serous | Carcinosarcoma | 51 | IB | DS | TP53 | p.X307 splice | c.919+1G>A | yes | 0.0075 | 42 | 5593 |
P22 | Serous | HGSC | 52 | IIIC | BROCA | TP53 | P.G244V | c.731G>T | yes | 0.0050 | 21 | 4166 |
P23 | Serous | HGSC | 56 | IIB | BROCA | TP53 | p.R342Tfs*4 | c.1023 1024insAC | yes | 0.0005 | 2 | 3835 |
P24 | Serous | HGSC | 57 | IB | DS | TP53 | P.C275F | c.824G>T | yes | 0.0169 | 97 | 5723 |
P25 | Serous | HGSC | 61 | IIA | DS | TP53 | P.V157F | C.469G>T | no | |||
P26 | Serous | HGSC | 62 | IIIB | DS | TP53 | P.R273C | c.817C>T | yes | 0.0037 | 22 | 5905 |
P27 | Serous | HGSC | 67 | IIIA1 | BROCA | TP53 | p.V274A | c.821T>C | yes | 0.0005 | 2 | 3938 |
P28 | Serous | HGSC | 68 | IVB | DS | TP53 | P.V143M | c.427G>A | no | |||
P29 | Serous | HGSC | 68 | IVB | DS | TP53 | p.S315Rfs*26 | c.942 954del | no | |||
P30 | Serous | HGSC | 71 | IIIC | DS | TP53 | P.H193Y | c.577C>T | yes | 0.0091 | 56 | 6130 |
P31 | Serous | HGSC | 72 | IIIC | BROCA | TP53 | P.S241F | C.722OT | yes | 0.0045 | 9 | 1999 |
P32 | Serous | HGSC | 73 | IIIC | DS | TP53 | p.X126 splice | c.376-2A>G | yes | 0.0002 | 1 | 4291 |
P33 | Serous | HGSC | 76 | IIA | BROCA | TP53 | P.P151H | c.452C>A | yes | 0.0962 | 372 | 3866 |
P34 | Serous | HGSC | 83 | IIB | DS | TP53 | p.S261Vfs*84 | c.780del | yes | 0.1832 | 926 | 5055 |
Abbreviation: DS, duplex sequencing; HGSC, high-grade serous carcinoma.
aNote that patient P19 had three tumor driver mutations.