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. 2022 Jul 27;8(10):1393–1403. doi: 10.1021/acscentsci.2c00058

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© 2022 The Authors. Published by American Chemical Society

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3-formyl-Chlide a (Chlide d) can be transformed into 3-formyl-Chlide b by combination of PhCAO with VanB and NADPH. (a) Reaction scheme to synthesize 3-formyl-Chlide a and the proposed route to C7-oxygenation by CAO. (b) The extracted ion chromatograms for the product formed when Chlide a or Chlide a with β-mercaptoethanol and heme was provided to PhCAO as a substrate. (c) The extracted ion chromatograms show that PhCAO can transform 3-formyl-Chlide a (Chlide d) into 3-formyl-Chlide b.