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. 2022 Oct 14;16:1012422. doi: 10.3389/fnana.2022.1012422

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Copyright © 2022 Hassan, Elnagar, Abdelrazik, Mahdi, Hamza, Elattar, ElNashar, Alghamdi, Al-Qahtani, Al-Khater, Aldahhan and ELdesoqui.

This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

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Immunohistochemical detection of cerebellar iNOS (A,B) C and N groups, respectively showed negative iNOS immunostaining. (C) AlCl₃ group showed strong positive iNOS immunostaining in most Purkinje cells (arrows). (D) AlCl₃ + N group showed weak positive immunostaining for iNOS in some Purkinje cells (arrows) (iNOS × 400). (E) Area% of iNOS positive immune reaction in cerebellar sections of all studied groups. n = 6 in each group. Data were prescribed as mean ± SD. The intergroup variation was conducted by Games-Howell post-hoc test. *p < 0.001 compared with C group. ^#p < 0.001 compared with AlCl_3. ^$p < 0.001 compared with AlCl₃.