FIGURE 1.

Procedure for creating the wound model. A, After anaesthesia, the hair on their back was shaved, and sterilised with 70% ethanol. B, An 8‐mm diameter full‐thickness skin defect wound was created on the back of the rats with a hole puncher (Kai disposable dermal biopsy punch 8 mm). C, The full‐thickness skin defect wound was to attached a polyethylene terephthalate bottle tap cut by electric heating saw (ILOHAS, natural mineral water, Coca‐Cola, Japan) with double‐sided tape (3M, Japan) to keep the tension of the surrounding skin constant