Fig. 1.

Glycine increased serum-induced killing of V. alginolyticus. A. Percent survival of V. alginolyticus 12G01 incubated with 100 mM glycine plus serum (0–100 μL) or without glycine B. Synergetic effects of 100 μL serum and glycine on viability of V. alginolyticus 12G01 were measured in a glycine dose-dependent manner (0–100 mM). C. Percent survival of V. alginolyticus 12G01 in the presence of 100 mM glycine or/and 100 μL serum for the indicated length of time. Results are displayed as mean ± standard errors of the means (SEM) (N = 3 technical replicates per sample), and statistically significant differences are identified. *, p < 0.05, **, p < 0.01. Each experiment was repeated independently at least three times.