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. 2022 Oct 27;22:455. doi: 10.1186/s12903-022-02511-9

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© The Author(s) 2022

Open AccessThis article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article's Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article's Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated in a credit line to the data.

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Fig. 4 — Differences in the percentages of 3 selected cariogenic (Streptococcus mutans, Lactobacillus sp., Actinomyces sp.) and 7 periodontal bacteria (Aggregatibacter actinomycetemcomitants, Tannarella forsythia, Porphyromonas gingivalis, Prevotella intermedia, Treponema, Denticola, Parvimonas micra, Fusobacterium nucleatum) relative to the total prokaryotic DNA at two time points – T0 (before bonding of fixed orthodontic appliances) and T1 (till the end of 7^th month after bonding of fixed orthodontic appliances) in 30 patients. Full circle, patient with deterioration of oral bacterial composition; empty circle, patient with unchanged or improved oral bacterial composition; statistical significance was calculated using the Wilcoxon matched-pairs signed-rank test