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. 2022 Oct 28;20:170. doi: 10.1186/s12964-022-00984-3

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© The Author(s) 2022

Open AccessThis article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article's Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article's Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated in a credit line to the data.

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Fig. 3 — TRIM21 interacts with MICALL2. A Silver staining of proteins immunoprecipitated (IP) with MICALL2-antibody with protein extracts from CRC cells followed by identification with LC–MS/MS. The red box indicates one of the most abundant bands as compared with IgG. B List of proteins was analyzed by scaffold 4 proteome software and their information is presented. C Coimmunoprecipitations were performed to validate the interaction between endogenous MICALL2 and TRIM21 in HCT8 cells. D The colocalization of TRIM21 (red) and MICALL2 (green) in HCT8 cells was assessed by laser-scanning confocal microscopy, respectively (scale bar = 10 μm). Nuclei are stained with DAPI (blue). E Schematic representation of TRIM21, MICALL2 and its mutants. F Co-IP of His-MICALL2 with Flag-tagged TRIM21 and their truncation mutants, or Flag-TRIM21 with His- tagged MICALL2 and their truncation mutants from HEK293 cells. Cells were subjected to immunoprecipitation with α-Flag or His antibody. GAPDH is a housekeeping protein control