Fig. 4.

TRIM21 destabilizes MICALL2. A The ubiquitination modification (left) and quantification (right) of endogenous MICALL2 was analyzed by immunoprecipitation with anti-MICALL2 antibody and western blotting with anti-ubiquitin antibody in Flag-TRIM21- overexpressed HCT116 or HCT8 and control cells. GAPDH was used as a loading control. B Western blotting was used to detect the expression of MICALL2 in the TRIM21-overexpressed HCT116 and HCT8 cell line (left) and the protein levels in different groups were compared to those of GAPDH (right). C Western blot analysis of MICALL2 in TRIM21- overexpressed HCT116 and HCT8 cells and control cells treated with MG132 (100 μg/mL) for up to 8 h. D Western blot analysis of MICALL2 in HCT116 transfected with plasmids expressing TRIM21 or control plasmid, then treated with CHX (100 μg/mL) for up to 9 h