Fig. 6.

MICALL2 targets Wnt/β-catenin signaling pathways. A Volcano plot analysis of transcript expression by RNA-seq of HCT116 cells stably infected with empty vector or MICALL2 expression plasmid. Transcripts in red were significantly upregulated, while those in green were significantly downregulated (logFoldChange > 1, adjust P < 0.05). B GO enrichment circle plots for DEGs between the HCT116 cells stably infected with MICALL2 expression plasmid or empty vector (left). The outer circle depicts the relative fold change, with blue and red dots representing characteristics that are down-regulated and up-regulated, respectively. The inner quadrants are colored based on the z-score and their surface is a function of the enrichment P-value. The associated tables present the GO term ID and function (right). C Western blotting was used to assess the expression of β-catenin, C-myc and Cyclin D1 in the MICALL2-overexpressed HCT116 cell line or MICALL2-silenced HCT8 (left) and the protein levels in different groups were compared to that of GAPDH (right). D The β-catenin levels in cytoplasmic and nucleic fraction of MICALL2-overexpressed HCT116 cell and or MICALL2-silenced HCT8 lysates were analyzed by Western Blot (left), and the β-catenin levels in cytoplasmic and nucleic fraction were normalized to GAPDH or Lamin B1, respectively. Cyto, cytoplasm; Nucl, nuclear