Table 1.

Outcomes assessed in the VascuFit study

Outcome measure	Data collection instrument	Telephone interview	Baseline appointment (T0)	Fitness check (T1)	Final appointment (T2)
Inclusion criteria a	Questionnaire (5 items)	x
Exclusion criteria a	Questionnaire (8 items)	x
Physical activity readiness	PAR_Q (7 items)		x
Physical activity	PA_Q (41 items)		x
General well-being	POMS (19 items)		x		x
General health	Medical interview b, physical examination		x		x
Body composition	BMI, bioelectrical impedance analysis		x		x
Cardiovascular function	BP, heart rate, ECG at rest and during exercise		x	x	x
Macrovascular endothelial function	baFMD		x		x
Microvascular endothelial function	SVA		x		x
Molecular endothelial function	endomiRs (15 species)		x		x
Cellular endothelial function	ECs, EPCs		x		x
Circulating cardiovascular risk factors	fasting blood glucose, LDL-, HDL- and total cholesterol, triglycerides, hs-CRP, sphingolipidome		x		x
Pulmonary function	Spirometry		x		x
Cardiorespiratory fitness	Spiroergometry with breath-by-breath gas analysis and capillary blood lactate		x	x	x

^a See methods section for details, ^b Medical interview including general health, chronic diseases, lifestyle and family history of cardiovascular diseases (14 items) and current medication (2 items per drug)

Abbreviations: baFMD Brachial-arterial flow-mediated dilation, BMI Body mass index, BP Blood pressure, ECG Electrocardiography, ECs Mature endothelial cells, endomiRs Set of microRNAs regulating key molecular pathways of endothelial cell function, EPCs Endothelial progenitor cells, HDL-cholesterol High-density lipoprotein cholesterol, hs-CRP High-sensitivity C-reactive protein, LDL-cholesterol Low-density lipoprotein cholesterol, PA_Q Physical activity questionnaire, PAR_Q Physical activity readiness questionnaire, POMS Profile of mood states questionnaire, SVA Static retinal vessel analysis