Table 2.
Primers used in this study.
| Primer | Sequence (5′–3′) a | Use |
|---|---|---|
| cpxD-S-F | CTGTCGACTCTTGTCTCGTCTCATCCAGCCACT | Amplification of cpxD upstream homology arms |
| cpxD-S-R | ATTCAGACAACGGCGCATTTATCCGAACTTTGTGAATTAGCCTCT | |
| cpxD-X-F | AGAGGCTAATTCACAAAGTTCGGATAAATGCGCCGTTGTCTGAAT | Amplification of cpxD downstream homology arms |
| cpxD-X-R | ATGCGGCCGCCGGTTTGCTTGGCTGACTGA | |
| cpxD-W-F | GCTAGTTTGGCTGCCTGCTC | Detection exterior of cpxD mutants |
| cpxD-W-R | TGTTCCGCAAATGAAATGGT | |
| cpxD-N-F | TTACCGTGCCGTTCGTGG | Detection interior of cpxD mutants |
| cpxD-N-R | GCAGCAACCGCATCTAATACAC | |
| cpxD-C-F | CCGCTCGAGTAATGCTTATCTGTTGAACCCTCCT | Amplification of cpxD |
| cpxD-C-R | AAGGAAAAAAGCGGCCGCTTAATAGGCACGAACGGCATTGGTC | |
| cpxD-F | GTATTCCGTCACGTGCCTTT | Detection the transcription of cpxD |
| cpxD-R | GCATTGGGAAACGCTGTAAT | |
| cpxC-F | ATTTCATTTGCGGAACAAGC | Detection the transcription of cpxC |
| cpxC-R | CGCATAAGCAATGCATCAAC | |
| cpxB-F | GCTACCAAGCCAAGCTCAAC | Detection the transcription of cpxB |
| cpxB-R | TTGCGGTTCGATTCCTTTAC | |
| cpxA-F | GGCAGTTTAACCGGTATGGA | Detection the transcription of cpxA |
| cpxA-R | CGAGAGTCACCTACCGCAAT | |
| 16SrRNA-F | CCATGCCGCGTGAATGA | Detection the transcription of 16SrRNA |
| 16SrRNA-R | TTCCTCGCTACCGAAAGAACTT | |
| cpxD-EMSA-F | TAATGCTTATCTGTTGAACCCTCCT | Amplification of cpxD promoter region for EMSA |
| cpxD-EMSA-R | CCCCAAAGAAAGGAGTAATCTAAGT | |
| rpoE-EMSA-F | TAAAAAGATAAGATAAGCGGTC | Amplification of rpoE promoter region for EMSA |
| rpoE-EMSA-R | AGTGTGTAACAAAAATGAAAAGT | |
| rpoD-EMSA-F | GCGGAAGAAAAGCAAGAGTTGGTCA | Amplification of rpoD promoter region for EMSA |
| rpoD-EMSA-R | TCCATAATTGTATCCGTTTTGTGTG |