KEY RESOURCES TABLE
| REAGENT or RESOURCE | SOURCE | IDENTIFIER |
|---|---|---|
| Biological samples | ||
| Human SCLC bronchoscopy (Tumor #1) | Vanderbilt University Medical Center | 236D |
| Human SCLC biopsy (Tumor #2) | Vanderbilt University Medical Center | 3359-PK |
| Chemicals, peptides, and recombinant proteins | ||
| Sodium succinate dibasic hexahydrate, ReagentPus, ≥99% | Sigma Aldrich | Cat# S2378 |
| Mouse anti-Tubulin beta 3 (TUBB3) Clone TUJ1 | BioLegend | Cat# 801213; RRID:AB_2728521 |
| Rhodamine phalloidin | Invitrogen | R415 |
| Donkey anti-mouse Alexa Fluor 488 | Invitrogen | Cat# A-21202; RRID:AB_141607 |
| Mounting medium: Aqua-Poly/Mount | Polysciences Inc. | 18606-20 |
| Mouse laminin 5% in PBS | Corning | 354232 |
| Critical commercial assays | ||
| Seahorse XF Cell Mito Stress Test | Agilent | 103015-100 |
| Deposited data | ||
| Raw and analyzed single cell data (human cell lines and tumors) | This paper | GEO: GSE193961 |
| RPM scRNA-seq and WGS | Ireland et al., 2020 | GEO: GSE149180 |
| TKO scRNA-seq | Wooten et al., 2019 | GEO: GSE137749 |
| Experimental models: Cell lines | ||
| Human: H69 cell line | ATCC | NCI-H69; RRID:CVCL_1579 |
| Human: H82 cell line | ATCC | NCI-H82; RRID:CVCL_1591 |
| Human: H524 cell line | ATCC | NCI-H524; RRID:CVCL_1568 |
| Human: H1048 cell line | ATCC | NCI-H1048; RRID:CVCL_1453 |
| Human: DMS53 cell line | ATCC | CRL-2062; RRID:CVCL_1177 |
| Human: DMS454 cell line | Sigma Aldrich | RRID:CVCL_2438 |
| Human: H841 cell line | ATCC | NCI-H841; RRID:CVCL_1595 |
| Human: CORL279 cell line | Sigma Aldrich | COR-L279; RRID:CVCL_1140 |
| Human: Ready-to-Assay™ SUCNR1 / GPR91 Succinate Receptor Chem-1 cell line | Millipore | HTS241RTA |
| Experimental models: Organisms/strains | ||
| Mouse: Rb1 fl/fl;p53 fl/fl;Myc (RPM) | Oliver laboratory | RRID: IMSR_JAX:029971 |
| Mouse: RB1 fl/fl; P53 fl/fl; P130 fl/fl | Stanford Research Animal Facility | TKO mouse model: PMID: 20406986 |
| Software and algorithms | ||
| R version 3.6.3 | The R Foundation | http://r-project.org |
| Factoextra version 1.0.7 | The R Foundation | https://cran.r-project.org/web/packages/factoextra |
| WGCNA version 1.70-3 | Langfelder and Horvath, 2008 | https://horvath.genetics.ucla.edu/html/CoexpressionNetwork/Rpackages/WGCNA/ |
| ParTI | Hart et al., 2015 | https://www.weizmann.ac.il/mcb/UriAlon/download/ParTI |
| Python version 3.7.6 | Python Software Foundation | http://python.org |
| matplotlib version 3.0.3 | Hunter, 2007 | http://matplotlib.org |
| Seaborn version 0.10.1 | Waskom, 2021 | https://seaborn.pydata.org/ |
| scanpy version 1.8.0 | Wolf et al., 2018 | https://github.com/theislab/scanpy |
| scVelo version 0.2.3 | Bergen et al., 2020 | https://github.com/theislab/scvelo/ |
| Velocyto version 0.17 | La Manno et al., 2018 | http://velocyto.org/ |
| Scrublet version 0.2.1 | Wolock et al., 2019 | https://github.com/swolock/scrublet |
| Numpy version 1.17.0 | Oliphant, 2006 | http://numpy.org |
| STAR | Dobin et al., 2013 | https://github.com/alexdobin/STAR |
| DropEst version 0.8.6 | Petukhov et al., 2018 | https://github.com/kharchenkolab/dropEst |
| BooleaBayes | Wooten et al., 2019 | N/A |
| Scipy version 1.5.3 | Virtanen et al., 2020 | https://www.scipy.org/ |
| Cell Transport Potential algorithm | This paper | https://zenodo.org/badge/latestdoi/426287007 |
| CellRank 1.5.1 | Lange et al., 2022 | https://github.com/theislab/cellrank |
| Code generated in this manuscript | This paper | https://zenodo.org/badge/latestdoi/426287007 |