REAGENT or RESOURCE Antibodies | SOURCE | IDENTIFIER |
---|---|---|
Antibodies | ||
Rat anti-Ly-6A, PerCP-Cyanine5.5 Monoclonal |
eBiosciences | Cat# 45-5981-82, RRID:AB_914372 |
Serian Hamster anti-CD3e, AF700 Monoclonal, clone 500A2 |
BD Biosciences | Cat# 557984, RRID:AB_396972 |
Rat anti-CD4, Pac Blue Monoclonal, clone RM4–5 |
BD Biosciences | Cat# 553050, RRID:AB_394586 |
Rat anti-CD8a, APC-Cy7 Monoclonal, Clone 53–7.6 |
BD Biosciences | Cat# 557654, RRID:AB_396769 |
Rat anti-CD44, PE-Cy7 Monoclonal, Clone IM7 |
Thermo Fisher Scientific | Cat# 25-0441-82, RRID:AB_469623 |
Armenian Hamster anti-CD69, PE Monoclonal |
BD Biosciences | Cat# 553237, RRID:AB_394726 |
anti-NK1.1, FITC Monoclonal, Clone PK136 |
Tonbo Biosciences | Cat# 35–5941, RRID:AB_2621722 |
Rat Anti-CD11b, Allophycocyanin Conjugated Monoclonal Antibody, |
BD Biosciences | Cat# 553312, RRID:AB_398535 |
Armenian Hamster Anti-CD11c Monoclonal Antibody, FITC Conjugated, Clone HL3 |
BD Biosciences | Cat# 553801, RRID:AB_395060 |
Rat ant-Ly-6G and Ly-6C, BUV395 Monoclonal, Clone RB6–8C5 |
BD Biosciences | Cat# 563849, RRID:AB_2738450 |
Rat ant-SiglecF, BV421 Monoclonal, clone E50–2440 |
BD Biosciences | Cat# 562681, RRID:AB_2722581 |
Rat Anti-Mouse MHC Class II (I-A / I-E), eFluor650NC Monoclonal, Clone M5/114.15.2 |
Thermo Fisher Scientific | Cat# 95-5321-42, RRID:AB_1603293 |
Armenian Hamster Anti-CD103 (Integrin alpha E), PE Monoclonal, clone 2E7 |
Thermo Fisher Scientific | Cat# 12-1031-82, RRID:AB_465799 |
Rat anti-CD64, PE/Cyanine7 Monoclonal |
BioLegend | Cat# 139314, RRID:AB_2563904 |
Rat Anti-IFN-gamma, Allophycocyanin Monoclonal, clone XMG1.2 |
BD Biosciences | Cat# 554413, RRID:AB_398551 |
Bacterial and virus strains | ||
Mycobacterium tuberculosis strain HN878 | BEI Resources, Manassas, VA | N/A |
Deposited data | ||
scRNA-seq, raw and analyzed data | This manuscript | GSE200639 |
scRNA-seq, raw and analyzed data | PMID: 34253059 | GSE150657 |
Experimental models: Organisms/strains | ||
C57BL/6J | The Jackson Laboratory | 000664 |
Software and algorithms | ||
Cellranger 3.1 | 10x Genomics | https://support.10xgenomics.com/ |
Seurat 3 | Stuart et al. (2019) | https://satijalab.org/seurat |
ggplot2 | Wickham (2016) | https://cloud.r-project.org/package=ggplot2 |
biomaRt | Durinck et al. (2009) | https://bioconductor.org/packages/biomaRt |
PANTHER | Mi and Thomas (2009) | http://www.pantherdb.org/ |