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. 2022 Oct 14;13:932008. doi: 10.3389/fpls.2022.932008

Figure 1.

Figure 1

Mutants in different cytokinin biosynthetic genes show differential effects on primary root length and meristem size. (A) Simplified version of the parallel cytokinin biosynthesis pathways. (B,C) Primary root phenotype and length quantification of the wild-type Col-0, the triple mutant ipt357, and the double mutants cyp753a1 cyp753a2 (cypa1a2) and ipt29. (D,E) Root meristem phenotype and size quantification of the assorted genotypes. (F) Quantification of some biologically active cytokinins in the assorted genotypes expressed as picomoles per 100 roots. Pictures were taken 7 days after stratification. Scale bars indicate (B) 1 cm, and (D) 100 μm. Asterisks indicate values significantly different from Col-0 in a (C,E) Student's t test and (D) Mann–Whitney U test [*p < 0.05, **p < 0.01, ***p < 0.001, ****p < 0.0001; n ≥(C) 13, (E) 24, (F) 6]. ADP/ATP, adenosine di/triphosphate; DMAPP, dimethylallyl diphosphate; tRNA, transfer ribonucleic acid; IPT, isopentenyltransferase; iPR, isopentenyladenosine; cZR, cis-zeatin riboside; tZR, trans-zeatin riboside; iP, isopentenyladenine; tZ, trans-zeatin; cZ, cis-zeatin.