Figure 2.

cZ induces CK signaling locally and affects root growth in a concentration-dependent manner. (A) Primary root length of the wild-type Col-0, and the ipt357, cypa1a2, and ipt29 multiple mutants grown for 7 days in media supplemented with 100 nM of iP, tZ, and cZ. Black asterisks indicate values significantly different from the Col-0 mock treatment and color asterisks indicate significant differences from the corresponding mock genotype in a one-way ANOVA test (***p < 0.001, ****p < 0.0001; n ≥ 13). (B) Root growth inhibitory effects of iP, tZ, and cZ on the genotypes mentioned in (A). (C) Cytokinin signaling reporter TCSn responses to different cytokinin treatments. (D) cZ dose-response treatment of Col-0 and the ipt29 double mutant. Primary root length after 7-day-old plants growing on media supplemented with different cZ concentrations. Dots indicate the average ± SD of at least 20 seedlings. (E) Primary root growth is reduced in ipt29 mutant 15 days after sowing.