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. 2022 Oct 14;13:1011669. doi: 10.3389/fendo.2022.1011669

Figure 5.

Figure 5

Cana mitigates ferroptosis induced by high glucose in cardiomyocytes. (A) The images and (B) C11-BODIPY fluorescence ratio of lipid ROS (Scale bar: 200 µm). C11-BODIPY represents the level of staining with the probe (unoxidized), while oxC11- BODIPY (oxidized) represents the level of lipid ROS. Contents of (C) MDA, (D) total iron and (E) Fe2+ in H9C2 cells. (F) Content of GSH; (G) Activity of GSH-Px in H9C2 cells. (H) Transcriptional level and (I) protein level of ferroptosis related genes examined by qPCR and western blotting assay. ROS: reactive oxygen species; MDA: malondialdehyde. ROS, reactive oxygen species; MDA, malondialdehyde; GSH, glutathione; GSH-Px, glutathione peroxidase; FTN-H, ferritin heavy-chain; xCT, cystine-glutamate antiporter; GPX4, glutathione peroxidase 4; GAPDH, glyceraldehyde-3-phosphate dehydrogenase. (*: P<0.05; **: P<0.01; ***: P<0.001; compared with CON group; #: P<0.05; ##: P<0.01; ###: P<0.001; compared with HG group; n.s.: no significance.).