Table 1.
qPCR Primer sequences.
| Gene | Forward sequence (5’- 3’) | Reverse sequence (5’- 3’) | ProductSize (bp) |
|---|---|---|---|
| Rat_TfR1 | CGGCTACCTGGGCTATTGTA | TTCTGACTTGTCCGCCTCTT | 84 |
| Rat_FPN | TCCTGGGCTTCGACTGTATC | CAAGTGAAGGCCACAGTTCC | 124 |
| Rat_FTN-H | GGCTGAATGCAATGGAGTGT | TCTTGCGTAAGTTGGTCACG | 186 |
| Rat_SLC7A11 | GGTGGTGTGTTTGCTGTCT | AGAGGAGTGTGCTTGTGGA | 101 |
| Rat_GPX4 | AATTCGCAGCCAAGGACATC | GGCCAGGATTCGTAAACCAC | 170 |
| Rat_GAPDH | CAATCCTGGGCGGTACAACT | TACGGCCAAATCCGTTCACA | 162 |
| Mouse_TfR1 | TTGGGTAGTTGGAGATTGCC | TGAGGTCTTTGGCTTCTGGT | 247 |
| Mouse_FPN | AAGCGGCCCACACTAAGAAA | AGGCAATGTCCCATGTTGGT | 237 |
| Mouse_FTN-H | CATCAACCGCCAGATCAACC | GTCATCACGGTCTGGTTTCTTTAT | 220 |
| Mouse_SLC7A11 | GGCACCGTCATCGGATCAG | CTCCACAGGCAGACCAGAAAA | 100 |
| Mouse_GPX4 | CGCGATGATTGGCGCT | CACACGAAAACCCCTGTACTTATCC | 175 |
| Mouse_GAPDH | GAAGGGCTCATGACCACAG | AGATCCACGACGGACACATT | 221 |
TfR1, transferrin receptor 1; FPN, ferroportin; FTN-H, ferritin heavy-chain; SLC7A11, Solute carrier family 7 member 11; GPX4, glutathione peroxidase 4; GAPDH, glyceraldehyde-3-phosphate dehydrogenase.