Table 2.
The antibodies and dilution ratio.
| Antibodies | Reacts with | Dilution ratio | Cat No | Manufacturers |
|---|---|---|---|---|
| anti-TfR1 | Rat, Mouse | 1:1000 | ab84036 | Abcam/UK |
| anti-FPN1 | Rat, Mouse | 1:500 | ab58695 | Abcam/UK |
| anti-FTN-H | Rat, Mouse | 1:1000 | ab75973 | Abcam/UK |
| anti-GPX4 | Rat, Mouse | 1:1000 | ab125066 | Abcam/UK |
| anti-xCT | Rat, Mouse | 1:1000 | ab175186 | Abcam/UK |
| anti-GAPDH | Rat, Mouse | 1:10000 | ab181602 | Abcam/UK |
| HRP labeled Goat anti rabbit IgG (H + L) | 1:50000 | BK-R050 | Bioker/China |
TfR1, transferrin receptor 1; FPN, ferroportin; FTN-H, ferritin heavy-chain; xCT, cystine-glutamate antiporter; GPX4, glutathione peroxidase 4; GAPDH, glyceraldehyde-3-phosphate dehydrogenase.