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. 2021 Oct 1;2(4):450–459. doi: 10.1016/j.bpsgos.2021.09.004

Figure 2.

Figure 2

CF-PC synaptic transmission is enhanced in patDp/+ mice. (A) Typical CF-EPSCs recorded in WT and patDp/+ PCs at a holding potential of −30 mV. (B) Paired CF stimulations with a 100-ms interval show enhanced CF-EPSC amplitudes but equivalent paired-pulse depression in patDp/+ PCs compared with WT PCs (patDp/+, n = 10; WT, n = 16). (C) Typical CF-EPSCs recorded at a holding potential of −70 mV. (D) Again, paired CF stimulations show enhanced CF-EPSC amplitudes and equivalent paired-pulse depression in patDp/+ PCs compared with WT PCs (patDp/+, n = 6; WT, n = 7, respectively). (E–G) Dual label immunohistochemistry stains for calbindin (PCs) and VGlut2 (CF presynaptic terminals) in WT and patDp/+ cerebellar tissue reveals CF terminal arrangement on PC dendrites. Quantifying the number of VGlut2 puncta per 100-μm lengths of dendrite shows increased density of CF inputs to both (F) large-caliber (>2 μm) primary dendrites (patDp/+, n = 49; WT, n = 45) and (G) fine dendritic branches (patDp/+, n = 28; WT, n = 34). Scale bar = 2 μm. ∗p < .05, ∗∗p < .01, ∗∗∗p < .005. Boxplots show mean line with interquartile range. Boxplot error bars show the least extreme of either the highest and lowest values or mean ± (1.5 × interquartile range). All data are shown as median ± SEM. CF, climbing fiber; EPSC, excitatory postsynaptic current; ns, not significant; PC, Purkinje cell; PPD, paired-pulse depression; WT, wild-type.