Fig. 1.

Analysis of transgenic human AβPP expression in rTg-D rats. Brain sections from 3 M old rTg-D rats were immunolabeled with the mouse monoclonal antibody mAbP2–1 to specifically detect human AβPP (green) and the rabbit polyclonal antibody NeuN to detect neurons (red). Transgene-encoded human AβPP expression was detected in neurons in cortex (A), hippocampus (C) and thalamus (E). Scale bars = 10 µm. Higher magnification images of the boxed regions show human AβPP expression in neurons of the motor cortex (B), CA1 region of the hippocampus (D) and the ventrolateral thalamic nucleus (F). Scale bars = 50 µm. (G) Immunoblot analysis of human AβPP expression in total brain homogenates from wild-type rats and rTg-D rats. (For interpretation of the references to color in this figure legend, the reader is referred to the web version of this article.)