Fig. 9.

Comparison of perivascular glial activation in the cortex of rTg-D CAA type-2 rats and rTg-DI CAA type-1 rats. Brain sections from 12 M WT rats (A,B), 18 M rTg-D rats (C,D) and 12 M rTg-DI rats (E,F) were stained for fibrillar amyloid (blue), immunolabeled with an antibody to collagen IV to identify cerebral microvessels (red) and immunolabeled with an antibody to GFAP to identify astrocytes (green) (A,C,E) or immunolabeled with an antibody to Iba-1 to identify microglia (green) (B,D,F). Scale bars = 50 µm. rTg-D CAA type-2 rats do not show increased numbers and activation of perivascular astrocytes and microglia, similar to WT rats. In contrast, rTg-DI CAA type-1 rats exhibit strong increases in perivascular reactive astrocytes and activated microglia in response to the prominent cerebral capillary amyloid. (For interpretation of the references to color in this figure legend, the reader is referred to the web version of this article.)