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Plackett–Burman design for Kocuria sp. RAM1 carotenoids optimization. (A) Carotenoids yield predicted value versus experimental value. (B) PBD factors that influence carotenoids yield. The orange and blue bars are corresponding to the positive and negative effect, respectively. A: Peptone (g/l), B: Yeast extract (g/l), C: Beef extract (g/l), D: NaCl (g/l), E: Glucose (g/l), F: MgSO4 (g/l), G: pH, H: Temperature, J: Agitation (rpm), K: Inoculum size (%), L: Incubation period (h).
