Table 2.

The experiment design of RSM for the four independent variables affecting Kocuria sp. RAM1 carotenoids.

Run	Factors				Carotenoid (µg/ml)
	A	B	C	D	Observed	Predicted
1	0	0	2	0	800.00	773.10
2	0	0	0	0	380.06	509.25
3	− 1	− 1	− 1	− 1	575.10	580.38
4	− 1	1	− 1	− 1	376.00	330.46
5	− 2	0	0	0	222.18	239.28
6	1	1	− 1	− 1	218.46	261.83
7	1	− 1	− 1	− 1	547.88	611.47
8	1	1	1	1	366.12	429.13
9	0	0	0	0	555.74	509.25
10	− 1	− 1	1	1	597.46	622.38
11	− 1	1	1	1	378.72	264.98
12	0	0	0	0	534.92	509.25
13	0	0	0	0	555.52	509.25
14	1	− 1	1	1	890.86	886.25
15	0	2	0	0	137.32	91.50
16	1	1	− 1	1	316.34	284.33
17	− 1	− 1	− 1	1	519.10	462.26
18	1	1	1	− 1	398.66	405.35
19	0	0	0	0	495.28	509.25
20	0	0	0	− 2	711.60	636.14
21	1	− 1	− 1	1	667.40	618.92
22	− 1	1	− 1	1	156.14	227.38
23	0	0	0	2	484.46	541.79
24	1	− 1	1	− 1	880.46	877.51
25	0	0	0	0	534.00	509.25
26	− 1	− 1	1	− 1	757.38	739.23
27	0	0	− 2	0	460.68	469.45
28	2	0	0	0	469.74	434.51
29	0	− 2	0	0	770.86	798.55
30	− 1	1	1	− 1	250.00	366.77

A: Peptone (g/l), B: Temperature (°C), C: Agitation (rpm), D: Inoculum size (%).