Fig. 6. Flies do not appreciably modulate egg-laying rates in all substrate choice environments.

(A) Eggs laid per fly. Each dot represents one fly. (B) Fraction of eggs on 0 mM with 95% confidence interval. Each dot represents one fly. Flies laid an average of 38, 36, 38, 36, 30, and 33 eggs per fly. (C and D) Mean egg-laying rate during the search period on a substrate as a function of time since visiting another substrate. The 90% confidence interval is shaded. (C) Ninety percent confidence intervals of 0 and 200 mM egg-laying rate curves for CS flies in a 0 mM versus 200 mM chamber in light and medium gray, respectively, for comparison [reproduced from (16)]. P values are calculated using the two-sided Wilcoxon rank sum test (Materials and Methods) and are comparing 0.8 and 1.4% for the indicated bin. Filled arrows indicate first bin past 5 s for which P > 0.05 (i.e., a quantitative estimate of when the curves merge). Filled arrow for 0 mM versus 200 mM is marked with a star because the 0 and 200 mM egg-laying rate curves do not merge within the plotted interval. (E) Mean locomotor speed aligned to egg deposition. In total, 831 and 795 eggs for 0.8% versus 0.8% and 1.4% versus 1.4%, respectively. (F) Mean egg-laying rate as in (C) and (D), but for chambers with the same option on both sides. (G) Fraction of time spent on 0.8% per fly. Each dot represents one fly.