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. 2022 Oct 28;8(43):eabn9016. doi: 10.1126/sciadv.abn9016

Fig. 6. NPAD could not form normally in Pabpn1-null oocytes.

Fig. 6.

(A) Diagram showing the strategy of Pabpn1 conditional knockout. (B) Immunohistochemistry results showed that Pabpn1 was specifically knocked out in oocytes of Pabpn1fl/fl;Gdf9-Cre mice. The arrow indicates the expression of PABPN1 in the oocyte nucleus. Scale bars, 20 μm. (C) Immunofluorescence and FISH results showing the distribution of PABPN1 and PA+ in WT and Pabpn1-deletion oocytes. (D) Statistical analysis of the size of PA+ in WT and Pabpn1-null oocytes. (E) Schematic diagram showing the injection of different forms of PABPN1 (WT, ΔRRM, R200A, and ΔNter) into the oocytes of Pabpn1fl/fl;Gdf9-Cre mice. (F) FISH and immunofluorescence results showing the localization of overexpressed PABPN1 (WT, ΔRRM, R200A, and ΔNter) and PA+ in Pabpn1-null oocytes. Scale bars, 20 μm. (G) Immunofluorescence results showing the distribution of PABPN1 and SRSF2 in WT and Pabpn1-deletion oocytes. (H) Statistical analysis of the size of SRSF2 foci in WT and Pabpn1-null oocytes.