FIG 1.

ctDNA content of liquid biopsies from 23,482 patients. (A) 94% of samples had some level of detectable tumor content. (B) Variable ctDNA shed was seen across a range of cancer types, listed in order of median ctDNA fraction; the most common cancer types in the cohort (n > 500) are given in bold. (C) Variant/signature detection is somewhat dependent on ctDNA fraction, with short variants and kinase fusions commonly detected at low ctDNA fraction, but higher ctDNA levels seen in cases with detection of genomic signatures (bTMB-H [≥ 10 mutations/Mb], MSI-H) or copy number alterations. Quartiles are indicated on the density plots with vertical lines. Variant types are arranged in order of median TF of samples. bTMB-H, high blood tumor mutational burden; ctDNA, circulating tumor DNA; MSI-H, high microsatellite instability; LBx, liquid biopsy; TF, tumor fraction.