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Western immunoblot of PsaA protein performed with the F1-1B MAb. Lanes: 1, S. pneumoniae strain R6 whole-cell antigen preparations; 2, protein standards; 3 and 4, lysates from two clones of E. coli INVαF′ transformed with the vector plasmid pCR2.1; 5 and 6, lysates from two clones of E. coli INVαF′ harboring the recombinant plasmid pCRNB2. Numbers on the left are molecular masses, in kilodaltons.
