Antibodies |
|
JIM7 |
http://www.plantprobes.net/index.php |
JIM7 |
LM6M |
http://www.plantprobes.net/index.php |
LM6M |
LM13 |
http://www.plantprobes.net/index.php |
LM13 |
Anti-rat-IgG-FITC |
Thermofisher |
31629; RRID: AB_228240
|
Anti-rat-IgG-HRP |
Thermofisher |
A10549; RRID: AB_2534047
|
|
Bacterial and virus strains |
|
Agrobacterium GV3101 pMP90 |
GoldBio |
CC-207 |
|
Chemicals, peptides, and recombinant proteins |
|
Calcofluor white |
Merck |
18909 |
Endo-arabinase |
Megazyme |
E-EARAB |
LR White Resin |
London Resin Company |
AGR1280 |
|
Experimental models: Organisms/strains |
|
Arabidopsis thaliana: col-0, qrt1, arad1 |
NASC; https://www.arabidopsis.org/portals/mutants/stockcenters.jsp
|
Col-0, qrt1, arad1, arad2 |
ARAD1-OE1 and ARAD1-OE2 |
This Manuscript |
ARAD1-OE |
Arad1/arad2 |
This Manuscript |
Arad1/2 |
Agrobacterium tumefaciens: GV3101 pMP90 |
https://www.goldbio.com |
CC-207 |
|
Oligonucleotides |
|
arad1 genotyping Fwd primer TATGTGTTCAGGGTGGAAAAGT |
This Manuscript |
N/A |
arad1 genotyping Rev primer GGGAGACTTGACGCCAGATT |
This Manuscript |
N/A |
arad2 genotyping Fwd primer TCGTTTATTTTGGTGGCAGTC |
This Manuscript |
N/A |
arad2 genotyping Rev primer CGCCTCAGCCGGGTCAAAA |
This Manuscript |
N/A |
Sail LB primer GCCTTTTCAGAAATGGATAAATAGCCTTGCTTCC |
This Manuscript |
N/A |
Fwd primer for amplification of ARAD1 coding sequence caccATGGCGCGTAAATCTTCCCTCCTCAAAC |
This Manuscript |
N/A |
Rev primer for amplification of ARAD1 coding sequence TTAAATGGAAGTGATAAGACCGGTTTGG |
This Manuscript |
N/A |
RT PCR primer for arad1 FWD: GCTCCTCCACAGTCCAAAAG |
This Manuscript |
N/A |
RT PCR primer for arad1 RED: ACGAGCTGCTACGAAAGGAA |
This Manuscript |
N/A |
RT PCR primer for ARAD1-OE FWD: GAGTTGAGGATCGCAACACA |
This Manuscript |
N/A |
RT PCR primer for ARAD1-OE REV: CGTAGCAGCTCGTCGATTCT |
This Manuscript |
N/A |
|
Recombinant DNA |
|
Plasmid: pENTR-D-TOPO |
Thermofisher |
K240020 |
Plasmid:PMDC32 |
Addgene |
32078 |
|
Software and algorithms |
|
FEBIO |
FEBio Software Suite |
https://febio.org |
Finite Element Stomatal Model |
GitHub |
https://github.com/woolfeh/stomasimulator; http://doi.org/10.5281/zenodo.6546037
|
ImageJ (Fiji) |
NIH (Public Domain) |
https://imagej.nih.gov/ij/ |