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. 2022 Jul 25;32(14):3170–3179.e4. doi: 10.1016/j.cub.2022.05.042
REAGENT or RESOURCE SOURCE IDENTIFIER
Antibodies

JIM7 http://www.plantprobes.net/index.php JIM7
LM6M http://www.plantprobes.net/index.php LM6M
LM13 http://www.plantprobes.net/index.php LM13
Anti-rat-IgG-FITC Thermofisher 31629; RRID: AB_228240
Anti-rat-IgG-HRP Thermofisher A10549; RRID: AB_2534047

Bacterial and virus strains

Agrobacterium GV3101 pMP90 GoldBio CC-207

Chemicals, peptides, and recombinant proteins

Calcofluor white Merck 18909
Endo-arabinase Megazyme E-EARAB
LR White Resin London Resin Company AGR1280

Experimental models: Organisms/strains

Arabidopsis thaliana: col-0, qrt1, arad1 NASC; https://www.arabidopsis.org/portals/mutants/stockcenters.jsp Col-0, qrt1, arad1, arad2
ARAD1-OE1 and ARAD1-OE2 This Manuscript ARAD1-OE
Arad1/arad2 This Manuscript Arad1/2
Agrobacterium tumefaciens: GV3101 pMP90 https://www.goldbio.com CC-207

Oligonucleotides

arad1 genotyping Fwd primer TATGTGTTCAGGGTGGAAAAGT This Manuscript N/A
arad1 genotyping Rev primer GGGAGACTTGACGCCAGATT This Manuscript N/A
arad2 genotyping Fwd primer TCGTTTATTTTGGTGGCAGTC This Manuscript N/A
arad2 genotyping Rev primer CGCCTCAGCCGGGTCAAAA This Manuscript N/A
Sail LB primer GCCTTTTCAGAAATGGATAAATAGCCTTGCTTCC This Manuscript N/A
Fwd primer for amplification of ARAD1 coding sequence caccATGGCGCGTAAATCTTCCCTCCTCAAAC This Manuscript N/A
Rev primer for amplification of ARAD1 coding sequence TTAAATGGAAGTGATAAGACCGGTTTGG This Manuscript N/A
RT PCR primer for arad1 FWD: GCTCCTCCACAGTCCAAAAG This Manuscript N/A
RT PCR primer for arad1 RED: ACGAGCTGCTACGAAAGGAA This Manuscript N/A
RT PCR primer for ARAD1-OE FWD: GAGTTGAGGATCGCAACACA This Manuscript N/A
RT PCR primer for ARAD1-OE REV: CGTAGCAGCTCGTCGATTCT This Manuscript N/A

Recombinant DNA

Plasmid: pENTR-D-TOPO Thermofisher K240020
Plasmid:PMDC32 Addgene 32078

Software and algorithms

FEBIO FEBio Software Suite https://febio.org
Finite Element Stomatal Model GitHub https://github.com/woolfeh/stomasimulator; http://doi.org/10.5281/zenodo.6546037
ImageJ (Fiji) NIH (Public Domain) https://imagej.nih.gov/ij/