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. 2022 Jun 20;57(12):1512–1528.e5. doi: 10.1016/j.devcel.2022.05.014

Figure 2.

Figure 2

Macrophages are required for the timely removal of apoptotic cardiomyocytes

(A–C) (A) Images of uninjured and injured Tg(myl7:h2b-GFP;myl7:mKateCAAX) ventricles. Cyan outlined zoom panel highlights condensed nuclei (white arrowheads). Images of TUNEL stained hearts 6 hpi in (B) Tg(myl7:h2b-GFP) and (C) Tg(myl7:GFP) larvae. White arrowheads, apoptotic cardiomyocytes/myocardium.

(D) Image of a propidium iodide (PI)-stained Tg(myl7:h2b-GFP) heart at 1 hpi. White arrowheads, necrotic debris.

(E) Images of uninjured and injured irf8+/+ and irf8−/−Tg(myl7:h2b-GFP) ventricles stained by TUNEL at 24 hpi. White arrowheads, TUNEL+ cells.

(F) Images of uninjured and injured Tg(myl7:h2b-GFP;csfr1a:NfsB-mCherry) ventricles stained by TUNEL per macrophage ablation model injury group at 24 hpi. Cyan arrowheads, macrophages; white arrowheads, TUNEL+ cells.

(G) TUNEL+ myocardial cells in uninjured and injured, irf8+/+ and irf8−/−Tg(myl7:h2b-GFP) ventricles, n = 15–29.

(H) TUNEL+ myocardial cells in uninjured and injured Tg(myl7:h2b-GFP;csfr1a:NfsB-mCherry) ventricles per macrophage ablation group, n = 10–12.

(I) z slice of LSFM-acquired z stack, at 9 hpi, showing internalized myocardial debris (white arrowheads) in a macrophage in a Tg(myl7:GFP;mpeg1:mCherry) larva, V, ventricle-surface. Scale bars, 50 μm for (A)–(F) and 10 μm for (I). All representative images are 3D LSFM shown as maximum intensity projections unless otherwise stated. ∗ p ≤ 0.05, ∗∗ p ≤ 0.01, ∗∗∗ p ≤ 0.001. Two-way ANOVA followed by Holm-Sidak’s post-hoc tests. Data are represented as mean ± SEM.