Figure 1.

Single-cell transcriptomics of thirsty Drosophila

(A) Schematic protocols for humidity preference assay. Flies were kept in vials with or without water for the indicated times, then given a T-maze choice between a humid and a dry chamber.

(B) Increasing dehydration converts humidity avoidance behavior into attraction. Attraction returns to avoidance with thirst quenching.

(C) Schematic of the process of single-cell transcriptomics analyses comparing flies from the four conditions in (B). Two independent samples were processed for each condition.

(D) Total number of cells obtained from each sample, after filtration of low-quality barcodes and doublets.

(E) Left: UMAP plot from first clustering step, and identification of seven main cell classes. Right: pie chart showing the number of cells obtained from each of these classes.

(F) UMAP plots showing sub-clustering of each of the seven cell classes shown in (E). Known cell types within each class are labeled. Kenyon cell labels represent known subtypes that innervate corresponding mushroom body lobes. Withing the monoaminergic cells labels are OA, octopaminergic; TA, tyraminergic; 5HT, serotonergic; DA, dopaminergic. Within other, IPCs, insulin-producing cells.

See also Figure S1.