| REAGENT or RESOURCE | SOURCE | IDENTIFIER |
|---|---|---|
| Chemicals, peptides, and recombinant proteins | ||
| Drierite, 8 mesh | ACROS Organics / Fisher Scientfic | Cat#10185130 |
| Schneider's culture medium | Gibco / Thermo Fisher Scientific | Cat#21720024 |
| d(–)-2-amino-5-phosphonovaleric acid | Sigma-Aldrich | Cat#A8054 |
| DNQX | Sigma-Aldrich | Cat#D0540 |
| Tetrodotoxin | Abcam | Cat#ab120054 |
| DPBS (calcium and magnesium free) | Gibco / Thermo Fisher Scientific | Cat#14190086 |
| Papain | Sigma-Aldrich | Cat#P4762 |
| Collagenase I | Sigma-Aldrich | Cat#C2674 |
| DAPI | Thermo Fisher Scientific | Cat#D1306 |
| BSA | New England Biolabs | Cat#B9000S |
| D-Serine | Tokyo Chemical Industry | Cat#S0033 |
| L-Serine | Tokyo Chemical Industry | Cat#S0035 |
| Glycine | Sigma-Aldrich | Cat#G8898 |
| N-methyl-D-aspartic acid | Sigma-Aldrich | Cat#M3262 |
| Acetylcholine | Sigma-Aldrich | Cat#A6625 |
| Adenosine Tri-Pphosphate | Sigma-Aldrich | Cat#A2383 |
| Glutamate | Sigma-Aldrich | Cat#G0355000 |
| Adenosine | Sigma-Aldrich | Cat#A9251 |
| Tetanus toxin | Sigma-Aldrich | Cat#T3194 |
| α,β-methylene adenosine 5’-diphosphate | Sigma-Aldrich | Cat#M3763 |
| 4-Methylcyclohexanol (98%) | Sigma-Aldrich | Cat#153095 |
| 3-octanol (99%) | Sigma-Aldrich | Cat#218405 |
| Critical commercial assays | ||
| Chromium Single Cell 3’ Library & Gel Bead Kit v2, 4 rxns | 10X Genomics | Cat#PN-120267 |
| LightCycler 480 Probes Master | Roche | Cat#4887301001 |
| RNeasy Mini Kit | Qiagen | Cat#74104 |
| SuperScript III First-Strand Synthesis SuperMix | Invitrogen | Cat#18080400 |
| Universal Probe Library | Roche | Cat#04683633001; Cat#04869877001 |
| LightCycler 480 Probes Master | Roche | Cat#4887301001 |
| Deposited data | ||
| Raw sequencing data and processed dataset | This study | GEO: GSE207799 |
| Drosophila melanogaster reference genome (dm6) and gene annotations (FlyBase, release 6.25, FB2018_06) | Thurmond et al.116 | https://flybase.org/ |
| Full Adult Female Brain (FAFB) transmission electron microscope (TEM) dataset | Zheng et al.85 | https://temca2data.org/ |
| Experimental models: Organisms/strains | ||
| Drosophila: 0273-GAL4 | Burke et al.117 and Gohl et al.118 | N/A |
| Drosophila: w; repo-GAL4, tub-GAL80ts; repo-GAL4 | S. Schirmeir & C. Klämbt | N/A |
| Drosophila: R86E01-GAL4 | Bloomington Drosophila Stock Center; Kremer et al.58 | RRID:BDSC_45914 |
| Drosophila: R85G01-GAL4 | Bloomington Drosophila Stock Center; Kremer et al.58 | RRID:BDSC_40436 |
| Drosophila: R66C08-GAL4 | Bloomington Drosophila Stock Center | RRID:BDSC_49412 |
| Drosophila: UAS-mCherry | NA | Lab stock |
| Drosophila: UAS-aayRNAi | Vienna Drosophila RNAi Center | RRID:FlyBase_FBst0454865 |
| Drosophila: InosRNAi | Vienna Drosophila RNAi Center | RRID:FlyBase_FBst0472636 |
| Drosophila: UAS-GstD1RNAi | Vienna Drosophila RNAi Center | RRID:FlyBase_FBst0475105 |
| Drosophila: UAS-CG33970RNAi | Vienna Drosophila RNAi Center | RRID:FlyBase_FBst0462630 |
| Drosophila: UAS-Socs36ERNAi | Vienna Drosophila RNAi Center | RRID:FlyBase_FBst0469597 |
| Drosophila: UAS-IrcRNAi | Vienna Drosophila RNAi Center | RRID:FlyBase_FBst0472971 |
| Drosophila: UAS-GstD9RNAi | Vienna Drosophila RNAi Center | RRID:FlyBase_FBst0475656 |
| Drosophila: UAS-Obp44aRNAi | Vienna Drosophila RNAi Center | RRID:FlyBase_FBst0464970 |
| Drosophila: UAS-Sod3RNAi | Vienna Drosophila RNAi Center | RRID:FlyBase_FBst0471232 |
| Drosophila: UAS-CG9377RNAi | Vienna Drosophila RNAi Center | RRID:FlyBase_FBst0464792 |
| Drosophila: UAS-Cyp28d1RNAi | Vienna Drosophila RNAi Center | RRID:FlyBase_FBst0470866 |
| Drosophila: UAS-Pcyt2RNAi | Vienna Drosophila RNAi Center | RRID:FlyBase_FBst0477620 |
| Drosophila: UAS-DratRNAi | Vienna Drosophila RNAi Center | RRID:FlyBase_FBst0480136 |
| Drosophila: UAS-CG33970 (osy) | Bernard Moussian; Wang et al.119 | N/A |
| Drosophila: UAS-aay | FlyORF | RRID:FlyBase_FBst0501667 |
| Drosophila: UAS-GstD9 | FlyORF | F004171 |
| Drosophila: UAS-Obp44a | FlyORF | F003929 |
| Drosophila: UAS-Sod3 | FlyORF | F003855 |
| Drosophila: UAS-Daao1 | Dai et al.70 | N/A |
| Drosophila: UAS-Shits1 | Kitamoto120 | Lab stock |
| Drosophila: UAS-Kir2.1 | Paradis et al.121 | Lab stock |
| Drosophila: UAS-TNT E | Sweeney et al.122 | Lab stock |
| Drosophila: UAS-GCamP7f | Dana et al.123 | RRID:BDSC_79031; RRID:BDSC_80906 |
| Drosophila: NMDAR1K558Q | Nigel Atkinson; Troutwine et al.74 | N/A |
| Drosophila: NMDAR1F654A | Nigel Atkinson; Troutwine et al.74 | N/A |
| Oligonucleotides | ||
| TTCGCGAGGATGAATACGAT | This study | SdhA – FWD |
| CACGAGAGCGTGTGCTTG | This study | SdhA – REV |
| AAAAAGCTCCGGGAAAAGG | This study | GAPDH – FWD |
| AATTCCGATCTTCGACATGG | This study | GAPDH – REV |
| CACCATGAAGAACGCTGTTG | This study | Obp44 – FWD |
| GCTTGTAGTCGGAGGCAGAG | This study | Obp44 – REV |
| CCGTTTCCACGACATTGAGT | This study | Drat – FWD |
| GTTAATGCCTTGATGGGGAAC | This study | Drat – REV |
| TGGCTCCCTTTATCCCAAG | This study | GstD9 – FWD |
| AAAAACAGGCGCTGATTGAT | This study | GstD9 – REV |
| CGGTGACTCCCTTACCGTAG | This study | GstD1 – FWD |
| TTTGGCCACCTCGAATGT | This study | GstD1 – REV |
| AAAAAGCCAGCAAACCAAAA | This study | Socs36E – FWD |
| AGGTGATGACCCATTGGAAG | This study | Socs36E – REV |
| TGCAATGGGTGGAATTCAG | This study | Irc – FWD |
| ACCATTTCGAAGCAGGAATC | This study | Irc – REV |
| CCCCTGAAAAACGTCTATGC | This study | aay – FWD |
| AGCTATCGTATTCGCCCAAA | This study | aay – REV |
| AGTGCTGTAATATCCCCGATAAAC | This study | CG9377 – FWD |
| CAGAGATCATGGCGTCCTC | This study | CG9377 – REV |
| ACCGCTCTTTATGGACTTTGAG | This study | Pcyt2 – FWD |
| GCATGGCCTTGTCATCGTA | This study | Pcyt2 – REV |
| Software and algorithms | ||
| CellRanger | 10X Genomics | https://support.10xgenomics.com/single-cell-gene-expression/software/overview/welcome; RRID:SCR_017344 |
| R | R Development Core Team, 2008 | http://www.R-project.org/; RRID:SCR_001905 |
| Seurat v3 | Hafemeister and Satija124 and Stuart et al.125 | https://satijalab.org/seurat/; RRID:SCR_016341 |
| DoubletFinder | McGinnis et al.126 | https://github.com/chris-mcginnis-ucsf/DoubletFinder; RRID:SCR_018771 |
| ZINB-WaVE | Risso et al.46 and Van den Berge et al.47 | https://github.com/drisso/zinbwave |
| DESeq2 | Love et al.48 | https://bioconductor.org/packages/release/bioc/html/DESeq2.html; RRID:SCR_015687 |
| edgeR | Robinson et al.49 and McCarthy et al.127 | https://bioconductor.org/packages/release/bioc/html/edgeR.html; RRID:SCR_012802 |
| ClusterProfiler | Yu et al.128 | https://github.com/YuLab-SMU/clusterProfiler |
| GOplot | Walter et al.129 | https://wencke.github.io/ |
| ScanImage 3.8 | Pologruto et al.130 | http://scanimage.vidriotechnologies.com |
| Fiji | NIH; Schindelin et al.131 | https://fiji.sc/ |
| FlyWire | Dorkenwald et al.84 | https://flywire.ai/ |
| Neuroglancer | Dorkenwald et al.84 | https://github.com/google/neuroglancer |
| CAVEclient | https://github.com/seung-lab/CAVEclient | |
| FAFBseg v.1.4 | https://github.com/navis-org/fafbseg-py | |
| Navis v.0.6 | https://github.com/navis-org/navis | |
| trimesh | https://github.com/mikedh/trimesh | |
| skeletor 1.1 | Au et al.132 | https://github.com/navis-org/skeletor |
| Blender 3.0 | Blender Community | https://www.blender.org/download/releases/3-0/ |
| GraphPad Prism 9 | GraphPad Software | https://www.graphpad.com/scientific-software/prism/; RRID:SCR_002798 |
| Adobe Illustrator | Adobe Systems | https://www.adobe.com/uk/products/illustrator.html; RRID:SCR_010279 |
| Other | ||
| 10 mm CellTrix strainer | Sysmex | Cat#04-0042-2314 |
| Fuchs-Rosenthal haemocytometer | VWR | Cat#631–1096 |
| Fisherbrand chromatography paper 180g/m2 460x570 mm | Fisher Scientific | Cat#15649494 |
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