Figure 3.

LSE is stimulus specific and dependent on recent threat history

(A) Left: schematic of the experimental paradigm in which a mouse was free to interact with a novel cricket tethered to the center of the threat zone (grey circle indicates the upper limit of the range of cricket at retreat onset). An example trace of a retreat to shelter is represented by the orange line, starting from the moment the mouse enters the regional limit of the tether point (orange circle). Right: positional traces for all mice and all bouts exhibiting returns into or near to shelter for each experimental group. Small grey circles indicated the position of the cricket at the onset of retreat for each bout.

(B) Top: raster plots of the onset times of all retreats for naive (green, n = 5) and LSE (brown, n = 6) mice. Middle: histograms of retreat onset times scaled to the first 4 min time bin. Bottom: line plots of the fraction of all bouts that result in a retreat, over time.

(C) Bar plot of escape probability to auditory stimuli in loom-naive (green) and LSE (brown) mice.

(D) Schematic showing the experimental paradigm for assessing the effect of prior exposure to a pre-test at either <0.2 h (orange) or 24–26 h (blue) before LSE and the subsequent total number of stimulus exposures (right).

(E) Pre-test escape probability of mice tested <0.2 h before or 24–26 h before LSE.

(F) Post-LSE escape probability for the <0.2 h and 24–26 h, and no pre-test experimental groups.∗∗, ∗∗∗ indicate p values of less than 0.01 and 0.001, respectively.