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. 2022 Oct 28;13:6420. doi: 10.1038/s41467-022-34137-4

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© The Author(s) 2022

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Fig. 3 — a DED maps contoured at ±3.5σ (blue and magenta correspond to the appearance and disappearance of density, contoured at 3.5σ). Dark state DED signals contoured at (±)3.5σ. The F_Dark1–F_Dark2 and F_Dark3–F_Dark2 exhibit marginal DED signals. The F_I1–F_Dark, F_I2–F_Dark, F_I3–F_Dark, and F_I4–F_Dark datasets show reproducible strong DED signals in the three different zones. The following domains undergoing photoinduced conformational changes are represented in oval shapes: Zones 1 (NTD), Zones 2 (NTE), and Zones 3 (Alpha-helices in CTD). b Difference electron density amplitudes calculated as described in the X-ray data refinement section of the “Methods”.