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. 2022 Oct 28;13:6421. doi: 10.1038/s41467-022-33908-3

Fig. 3. NSP1 and NSP2 control nutrient regulation of symbiosis signaling and AMF colonization.

Fig. 3

Nutrient regulation of symbiosis signaling in 16-day-old barley (a) and M. truncatula (b) lateral roots grown under different nutrient regimes, responding to SmLCO, NS-LCO, or CO4. Calcium spiking (%) indicates the number of cells responding with calcium oscillations, with 0 indicating no calcium oscillations observed. c A heatmap showing the expression of M. truncatula and barley NSP1 and NSP2 homologs upon N- and/or P- starvation. +P-N represents the comparison of gene expression under +P−N to +P + N ( + P−N/ + P + N), similarly −P + N and −P-N representing −P + N/ + P + N and −P−N/ + P + N. d The percentage of M. truncatula atrichoblasts on lateral roots showing calcium oscillations in wild type and the nsp1-1/nsp2-2 double mutant, grown under nutrient replete or deplete conditions. e AMF colonization of M. truncatula mutants grown under P-limited conditions, measured at 3 weeks post inoculation. n = 5 biologically independent samples. f Root-length colonization of barley wild type, nsp1a, and nsp2 mutants grown under P-limited conditions, measured at 7 weeks post inoculation. n = 5–6 biologically independent plants. Total: total colonization; A: arbuscules. p-values for colonization levels were determined by a one-sided Wilcoxon test. **p < 0.01; *0.01 < p < 0.05.