Fig. 5. DiLiCre2.0 mouse model is an efficient optogenetic tool to induce oncogenic cell transformations in vivo.

a Schematic representation of the breeding setup to generate the DiLiCre2.0;HrasV12-eGFP transgenic mouse model. LSL: Lox-Stop-Lox. b Schematic drawing showing the experimental setup for the photoactivation of the hair follicles. c Representative longitudinal images of the mouse ear skin upon DiLiCre2.0 photoactivation (HFs, mouse 1). The upper row shows widefield tissue overview images of the ear skin where the blood vessel pattern (black lines) and hair follicles (small white spots) can be identified. Within each image, the areas photoconverted are boxed using white dotted squares. The bottom row displays two-photon images corresponding to the yellow boxed areas. Within these images, the photoactivated regions are annotated. n = 3 biologically independent experiments/mice were performed with similar results. d Quantification of HrasV12-eGFP fluorescence over time in 405 nm light exposed and non-exposed regions. Control positions were selected randomly at least 150 µm away from the photoconverted areas. This experiment was performed in three mice and each graph shows the data of one individual. All biological replicates shown similar results. Between the two arms, the entire curves were compared statistically and the P values were determined by permutation test adapted from Elso et al. (2004). Source data are provided as a Source Data file.