Figure - PMC

Skip to main content

An official website of the United States government

Here's how you know

Here's how you know

Official websites use .gov
A .gov website belongs to an official government organization in the United States.

Secure .gov websites use HTTPS
A lock ( ) or https:// means you've safely connected to the .gov website. Share sensitive information only on official, secure websites.

View full-text article in PMC

. 2022 Oct 28;13:6423. doi: 10.1038/s41467-022-34165-0

Search in PMC
Search in PubMed
View in NLM Catalog
Add to search

© The Author(s) 2022

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

PMC Copyright notice

Fig. 5 — a, e MCCs are labeled with α-tubulin::LifeAct-RFP (pseudo-colored in green in composite image and pseudo-colored in gray as separate channel) and myosin II in goblet cells is labeled by myosin II intrabody SF9-3xGFP (pseudo-colored in magenta in composite image and pseudo-colored in fire as separate channel). a Image sequence of epithelial junction remodeling during MCC integration. Scale bar: 5 μm. Orange arrowheads mark the onset of remodeling. b Normalized junctional myosin II intensity (magenta) and junction length (purple) during remodeling from a. Orange and blue bars mark the start of remodeling and normalized myosin II intensity equaling one, respectively. c Normalized MCC F-actin intensity (green) and junction length (purple) during remodeling from a. Orange bar marks the start of remodeling. d Normalized junctional myosin II (magenta) and normalized MCC F-actin (green) intensities during remodeling from a. Orange and blue bars mark the start of remodeling and normalized myosin II intensity equaling one, respectively. e Orthogonal (XZ) projections of a. Yellow arrows depict MCC contact with the epithelial vertices. Scale bar: 5 μm. f Vertex retraction during junction remodeling. MCC expresses α-tubulin::LifeAct-GFP (pseudo-colored in green) and goblet cells express nectin::utrophin-RFP (pseudo-colored in magenta). Orange arrowheads mark the onset of remodeling. Cyan arrowheads mark the loss of MCC contact with the vertices. Scale bar: 5 μm. White dotted line outlines the MCC contour. Yellow arrows depict contact with the epithelial vertices. g MCC F-actin fluorescence intensity (green) and junction length (purple) during junction collapse (indicated by the orange bar) and retraction (indicated by cyan bar) from f. h, i Stability-diagram of junction collapse in the normalized junction length-tension phase space from h in silico and i in vivo. The magenta triangles mark the junction length vs. line tension of collapsed junctions, while cyan squares represent non-collapsed junctions, when perturbed h by line tension changes (in silico) and i by MCC remodeling (in vivo). (See Supplementary Note 1). j Distribution of junction length and tension for collapsed and non-collapsed junctions in vivo showing stronger sensitivity of junction collapse to the initial length than to tension (shown in the inset). k Schematics representing the multi-step, cooperative process of junction remodeling.