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. 2022 Oct 28;13:6429. doi: 10.1038/s41467-022-34153-4

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© The Author(s) 2022

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Fig. 3 — a Interaction between Dt2 and GmAgl22 in the yeast two-hybrid assay. Transformed yeast cells are grown on DDO (Trp/Leu) or QDO (Trp/Leu/His/Ade) synthetic dropout medium. The number at the top indicates three serial dilutions. AD, GAL4 activation domain; BD, GAL4 DNA-binding domain. b BiFC analysis of the interaction between Dt2 and GmAgl22. Scale bars, 10 mm. cYFP C-terminal portion of YFP. nYFP N-terminal portion of YFP. c Co-IP analysis of the protein interactions between Dt2 and GmAgl22. Flag-tagged GmAgl22 is co-transformed with HA-tagged Dt2 into Arabidopsis protoplasts. d Interaction between GmSoc1a and GmAgl22 in the yeast two-hybrid assay. e BiFC analysis of the interaction between GmSoc1a and GmAgl22. Scale bars, 10 mm. f Co-IP analysis of the protein interactions between GmSoc1a and GmAgl22. g Phenotypic comparison between W82 and GmSoc1a^CR lines. Scale bars, 20 cm. h Branch number statistic in W82 and GmSoc1a^CR lines (n = 20 biologically independent plants). i Phenotypic comparison between DN50 and GmAgl22^OE lines. Scale bar, 10 cm. j Branch number statistic in DN50 and GmAgl22^OE lines (n = 10 biologically independent plants). All the data in the graphs represent the mean ± SEM. Statistical significance is determined using a two-sided t-test. For (b, e), at least 5 independent cells are observed and a representative result is shown. For (c, f), at least 3 independent replicates are performed and a representative result is shown. Source data are provided as a Source Data file.