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. 2022 Oct 28;13:6274. doi: 10.1038/s41467-022-33786-9

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© The Author(s) 2022

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Fig. 5 — a Immunoprecipitation assay showing the interaction between endogenous CARM1 and members of ACTR in control and exon7-deleted MKN1 cell lines and RT-PCR analysis showing expression of LRRFIP2 variants. b Immunoprecipitation assay showing the interaction between endogenous CARM1 and members of ACTR in LRRFIP2 variants 2 and 3-overexpressing MKN28 cell lines. c Immunoblot analysis showing expression of H3R17me2a and ACTR following knockdown of ACTR in MKN1 cells. d RT-PCR analysis showing expression of SERPINE1 and ACTR following knockdown of ACTR in MKN1 cells. e qChIP analysis of the human SERPINE1 promoter with antibodies to CARM1, H3R17me2a in MKN1 cells following control and ACTR siRNA transfection. f Control and ACTR-knockdown MKN1 cells were transfected with SERPINE1 promoter (−1500/+500) and then assayed for luciferase activity. g Transwell migration assay and Matrigel invasion assay of MKN1 cells following knockdown of ACTR (left) and bar graphs showing number of invaded and migrated cells (right), respectively, following staining with crystal violet. Original magnification, _40X. Scale bar, 0.5 mm. h qRT-PCR result showing the relative mRNA expression of SERPINE1, CARM1, and ACTR. All P values were calculated by unpaired two-tailed Student’s t tests. a–c The representative results were obtained from at least three independent experiments. d–f, h Data are representative mean ± SD of three independent samples (N = 3). g Data are representative mean ± SD of three independent experiments (N = 3). Source data are provided in the Source Data file.