Table 1.
Constituents of the Nephrin/Neph1 interactome determined by meAP-MS, together with annotation of subcellular localization and primary function(s) indicated in literature and public databases
| ID | Acc. no. | Name | Topology/localization | Function |
|---|---|---|---|---|
| NPHN | Q9R044 | Nephrin | 1 TM | Cell adhesion, scaffold |
| KIRR1 | Q6X936 | Neph1 | 1 TM | Cell adhesion, scaffold |
| ANPRA* | P18910 | ANPRA | 1 TM | Receptor-mediated signaling, guanylyl cyclase |
| ANPRC* | P41740 | ANPRC | 1 TM | Receptor-mediated signaling |
| ROBO2 | Q7TPD3 | Roundabout homolog 2 | 1 TM | Receptor-mediated signaling |
| MERTK* | P57097 | MerTK | 1 TM | Receptor kinase |
| PTPRO* | E9Q612 | R-PTP-O | 1 TM | Receptor phosphatase, cell adhesion |
| FYN | Q62844 | Tyr-kinase Fyn | Membrane anchored (lipidation) | Kinase |
| DAAM2* | Q80U19 | DAAM2 | Cytoplasmic/membrane associated | Signaling |
| RRAS* | D3Z8L7 | R-Ras | Membrane anchored (lipidation) | Signaling, small GTPase |
| RRAS2* | P62071 | R-Ras2 | Membrane anchored (lipidation) | Signaling, small GTPase |
| DEND | P50617 | Dendrin | Membrane associated/nuclear | Signaling, apoptosis |
| FZD2* | Q08464 | Frizzled-2 | 7 TMs | GPCR |
| ACKR3* | O89039 | Atypical chemokine receptor 3 | 7 TMs | GPCR |
| MAGI1 | Q6RHR9 | MAGI-1 | membrane associated | Scaffold |
| MAGI2 | O88382 | MAGI-2 | membrane associated | Scaffold |
| A4* | P08592 | Amyloid precursor protein | 1 TM | APP, cell adhesion |
| ITM2B* | Q5XIE8 | Integral membrane protein 2B | 1 TM | APP regulation |
| NICA* | Q8CGU6 | Nicastrin | 1 TM | APP regulation |
| LSR* | Q9WU74 | Lipolysis-stimulated lipoprotein receptor | 1 TM | Cell adhesion |
| BCAM* | Q9ESS6 | Basal cell adhesion molecule | 1 TM | Cell adhesion |
| PKP2* | Q99959 | Plakophilin-2 | Membrane associated | Cell adhesion |
| DSG1* | Q02413 | Desmoglein-1 | 1 TM | Cell adhesion |
| ITAV* | P43406 | Integrin alpha-V | 1 TM | Cell adhesion |
| CLD15* | D3ZQJ0 | Claudin-15 | 4 TMs | Cell adhesion |
| AT2B1* | P11505 | PMCA1 | 10 TMs | Transporter |
| CTL2* | B4F795 | Choline transporter-like protein 2 | 10 TMs | Transporter |
| MGT5A* | Q08834 | N-acetyl-glucosaminyl-transferase V | 1 TM | N-acetyl-glucosaminyltransferase |
| ERLN2* | B5DEH2 | Erlin-2 | ER, 1 TM | ERAD |
| ERLEC* | Q96DZ1 | ER lectin 1 | ER lumen | ERAD |
| RCN1* | Q05186 | Reticulocalbin-1 | ER lumen | Calcium binding |
Accession codes refer to the UniProtKB/Swiss-Prot database; underlining refers to previous report(s) on podocyte-related function(s), asterisks denote proteins that have not been reported to interact with Nephrin or Neph1 before.