Fig. 8.

GYY4137 and HMPSNE treatments on 3-MST knock-down adipocytes. A Representative immunoblot showing the efficiency of 3-MST knockdown in pre-adipocytes (day 0) and mature adipocytes (day 7) and corresponding densitometry analysis. One-way ANOVA followed by Bonferroni post-test. *p < 0.05 compared to day 0 shCTR; #p < 0.05 compared to day 7 shCTR. B Oil Red O staining on control silenced (shCTR) or 3-MST silenced (sh3-MST) adipocytes treated with GYY4137 (1–3 mM). Data refer to mean values of N = 5 independent experiments ± SEM. *p < 0.05 and **p < 0.01 show significant differences in the measured values on day 7 (differentiated cells) compared to the initial values (non-differentiated values) on day 0; ^#p < 0.05 and.^##p < 0.01 show significant effect of 3-MST silencing or the H₂S donor (GYY4137) compared to the corresponding control values