Fig. 9.

Effect of 3-MST pharmacological inhibition or knockout on bioenergetic parameters of inguinal white adipose tissue. Oxygen consumption rate measurements in inguinal white adipose tissue (iWAT) of WT and Mpst^−/.⁻ mice in the presence of glucose (A) or palmitate (B) determined using a Seahorse Flux Analyzer. The assay protocol consisted of the subsequent addition of 50 µM oligomycin, 25 µM FCCP, and 20 µM rotenone/antimycin A, as described in the “Methods” section. Data refer to mean values of at least N = 5 independent experiments ± SEM. *p < 0.05 and **p < 0.01 show significant differences