Fig. 3.

Senescent cells accumulated in skeletal muscle following mechanical overload (MOV). (a) Representative image for senescence-associated beta-galactosidase (SA β-Gal) staining 14 days following MOV in an old mouse. (b) Quantification of SA β-Gal + cells per 10⁵ μm² in adult (blue circles) and old (red triangles) mice following sham surgery (controls) and 7 and 14 days of MOV induced by synergist ablation surgery. (c) Representative image of p21 immunohistochemistry in the plantaris 14 days following MOV in an old mouse. (d) Quantification of p21 + cells per 10⁵ μm² in adult (blue circles) and old (red triangles) mice following sham surgery (controls) and 7 and 14 days of MOV induced by synergist ablation surgery. N = 6–8/group. *Significance versus sham controls for a given age group. ^Significance versus adult mice for a given time point