Table 2.
Primers and TaqMan miRNA assays
| Target gene | Forward | Reverse |
|---|---|---|
| ADIPOQ | GAGATGGACGGACGGAGTCCTTTAGG | CTGGTCATGTTTGTGAAGCTCCC |
| β-actin | TGAGAGGGAAATCGTGCGTG | TGCTTGCTGATCCACATCTGC |
| CD206 | TATGGAATAAAGACCCGCTGAC | TGCTCATGTATCTCTGTGATGCT |
| GAPDH | GGCACAGTCAAGGCTGAGAATG | ATGGTGGTGAAGACGCCAGTA |
| GLUT4 | CATTCCTTGGTTCATCGTG | ATAGCCTCCGCAACATAC |
| IL-1β | AGATGATAAGCCCACTCTACAG | ACATTCAGCACAGGACTCTC |
| IL-6 | TGCAATAACCACCCCTGACC | GTGCCCATGCTACATTTGCC |
| IL-8 | GGACAAGAGCCAGGAAGAAA | CCTACAACAGACCCACACAATA |
| IL-10 | AGGCATTCTTCACCTGCTCC | AAGACCCAGACATCAAGGCG |
| MCP1 | GGCTGAGACTAACCCAGAAAAG | GGGTAGAAACTGTGGTTCAAGAG |
| NF-kB | ACAGCTGGATGTGTGACTGG | TCCTCCGAAGCTGGACAAAC |
| p21 | CCATCCCTCCCCAGTTCATT | AAGACAACTACTCCCAGCCC |
| SIRT1 | TGTTTCCTGTGGGATACCTGA | TGAAGAATGGTCTTGGGTCTTT |
| TNF-α | AAGCCTGTAGCCCACGTGTA | GGCACCACTAGTTGGTGGTCTTTG |
| TGF-b | CCCAGCATCTGCAAAGCT | GTCAATGTACAGCTGCCGCA |
| IL-1α | CTTTCCCTGCCTGACCTTATT | GAATGAAGCTACTGCCCTACTC |
| PPAR-g | TGTGGGGATAAAGCATCAGGC | CCGGCAGTTAAGATCACACCTAT |
| CD163 | ACTGCAAGAACTGGCAATGG | CCATGCTTCACTTCAACAGG |
| TaqMan assay ID | ||
| miR-21 | ID00397 | |
| miR-146a | ID000468 | |
| RNU44 | ID001094 |