Table 3.
Primary antibodies
| Antibodies | Host* | Dilution | Source |
|---|---|---|---|
| AKT | R | 1:1000 | Cell Signaling Technology/9272 |
| α-tubulin | R | 1:1000 | Cell Signaling Technology/ 2144 |
| β-actin | M | 1:200 | Santa Cruz Biotechnology/sc-47778 |
| IKB-α | R | 1:1000 | Cell Signaling Technology/9242 |
| IL-1β | R | 1:1000 | Cell Signaling Technology/12242 |
| IRS1 | M | 1:200 | Santa Cruz Biotechnology/sc-8038 |
| JNK | M | 1:200 | Santa Cruz Biotechnology/sc-7345 |
| p21 | R | 1:1000 | Cell Signaling Technology/2947 |
| pAKT (Ser473) | R | 1:2000 | Cell Signaling Technology/4060 |
| pJNK | M | 1:100 | Santa Cruz Biotechnology/sc-6254 |
| SIRT1 | R | 1:1000 | Cell Signaling Technology/2496 |
*M mouse, R rabbit