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. 2022 Oct 26;3(4):101785. doi: 10.1016/j.xpro.2022.101785

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© 2022 The Authors

This is an open access article under the CC BY-NC-ND license (http://creativecommons.org/licenses/by-nc-nd/4.0/).

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Agarose gel electrophoresis of the diagnostic digestion of LentiV_Blast-BRD4-L plasmids

Red arrows indicate miniprep of successful clones (#1–#3). Black arrow indicates a failed clone (#4). White arrows indicate that the isolated clones presented empty LentiV_Blast vectors, with no BRD4-L inserts (#5-#6). The reference bands for estimating the expected band sizes after diagnostic digestion (8,229 bp and 2,672 bp) have been identified on the ladder as 2,000 and 7,000 base pairs (bp). Reference ladder used is GeneRuler 1 kb Plus DNA Ladder (Thermo Scientific™, cat# SM1334), 10 μL were added to each side of the gel.