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. 2022 Oct 26;3(4):101785. doi: 10.1016/j.xpro.2022.101785

Figure 7.

Figure 7

Overexpression of BRD4-L in mammalian cells

ProteinSimple Wes blots show that the mammalian cell lines Ovcar4, FT-190-TAg and FT-194-TAg when infected with the lentiviral plasmid LentiV_Blast-BRD4-L successfully overexpressed BRD4-L, after selection with Blasticidin (Left panel). A consistent amount of protein was assayed by Wes for each cell line pair (parental and overexpression), as can be confirmed by the loading control Vinculin (Right panel). Antibodies used: Rabbit monoclonal anti-BRD4 long isoform, Cell Signaling, cat# 13440 – dilution 1:50; Rabbit monoclonal anti-Vinculin, Cell Signaling, cat# 13901 – dilution 1:50. ProteinSimple Wes 66–440 kDa separation module (cat# SM-W008).