REAGENT or RESOURCE	SOURCE	IDENTIFIER
Antibodies
Rabbit monoclonal anti-BRD4 long isoform (Dilution 1:50)	Cell Signaling Technology	Cat# 13440; RRID: AB_2687578
Rabbit monoclonal anti-Vinculin (Dilution 1:50)	Cell Signaling Technology	Cat# 13901; RRID: AB_2728768
Bacterial and virus strains
NEB 5-alpha Competent E.coli (High Efficiency)	New England Biolabs	Cat# C2987H
Chemicals, peptides, and recombinant proteins
Betaine solution (5 M)	Sigma-Aldrich	Cat# B0300-5VL
Ampicillin antibiotic, sodium salt 25 g	VWR	Cat# 71003-352
Restriction enzyme - BamHI	New England Biolabs	Cat# R0136S
Restriction enzyme - EcoRI	New England Biolabs	Cat# R0101S
Restriction enzyme - MluI	New England BioLabs	Cat# R0198S
NEBuffer™ 3:1 (Provided with restriction enzyme)	New England Biolabs	Cat# B7203S
Luria Broth Base	Invitrogen	Cat# 12795-084
SOC Outgrowth Medium	New England Biolabs	Cat# B9020
Molecular Biology Grade Agarose LE	Thomas Scientific	Cat# C9961-57
Phusion™ High-Fidelity DNA Polymerase (2 U/μL)	Thermo Scientific™	Cat# F-530L
Phusion™ G-C Buffer (Provided with Polymerase)	Thermo Scientific™	Cat# F-530L
10 mM dNTP Mix	Invitrogen	Cat# 100004893
Cold Fusion Cloning Kit with Competent Cells	System Biosciences	Cat# MC010A-1
QIAquick Gel Extraction Kit	QIAGEN	Cat# 28704
QIAprep Spin Miniprep Kit	QIAGEN	Cat# 27106
CompactPrep Plasmid Maxi Kit	QIAGEN	Cat# 12863
Blasticidin antibiotic, S HCl (10 mg/mL), liquid	Gibco, Fisher Scientific	Cat# A1113903
Critical commercial assays
ProteinSimple Wes 66–440 kDa separation module	ProteinSimple (Bio-Techne)	Cat# SM-W008
Deposited data
NCBI Reference Sequence for assembly NM_058243.3	NCBI/NIH	https://www.ncbi.nlm.nih.gov/nuccore/NM_058243.3
Oligonucleotides
Cold fusion cloning BRD4-L forward primer: 5′ GGTGTCGTGACG CGGCTATGTCTGCGGAGAGCGG 3′	This paper	CF-BRD4-L_pLV-F
Cold fusion cloning BRD4-L reverse primer: 5′ CCATGGTGGCGGATCCC TAGGTGCGCTCAGAAAAGA 3′	This paper	CF-BRD4-L_pLV-R
Recombinant DNA
LentiV_Blast mammalian stable overexpression plasmid	Addgene; (Tarumoto et al., 2020)	Cat# 111887; RRID: Addgene_111887
pcDNA5-Flag-BRD4-WT transient expression plasmid	Addgene, a gift from Dr. Kornelia Polyak (Shu et al., 2016)	Cat# 90331; RRID: Addgene_90331
LentiV_Puro mammalian stable overexpression plasmid	Addgene, a gift from Christopher Vakoc (Tarumoto et al., 2020)	Cat# 111886; RRID: Addgene_111886
pLentiTRE/rtTA inducible stable overexpression plasmid	A gift from Trudy Oliver (Bieniasz et al., 2015)	N/A
LentiV_Blast-BRD4-L stable overexpression plasmid	This paper	LentiV_Blast-BRD4-L
LentiV_Puro-BRD4-L stable overexpression plasmid	This paper	LentiV_Puro-BRD4-L
pLentiTRE/rtTA-BRD4-L inducible overexpression plasmid	This paper	pLentiTRE/rtTA-BRD4-L
Software and algorithms
GenBank	NCBI/NIH	https://www.ncbi.nlm.nih.gov/genbank/ RRID: SCR_002760
Oligo Calculator, version 3.27	Northwestern University (Kibbe, 2007)	http://biotools.nubic.northwestern.edu/OligoCalc.html
Image Lab, version 6.0.1	Bio-Rad	RRID: SCR_014210
Compass software for SW, version 4.0.0	ProteinSimple (Bio-Techne)	https://www.proteinsimple.com/compass/downloads/
Other
UltraPure™ DNase/RNase Free distilled water	Invitrogen™	Cat# 10977015
GeneRuler 100 bp Plus DNA Ladder	Thermo Scientific™	Cat# SM0323
GeneRuler 1 kb Plus DNA Ladder	Thermo Scientific™	Cat# SM1334
DS-11+Spectrophotometer	DeNovix	N/A
Master Cycler Pro thermocycler	Eppendorf	N/A
Mini-sub Cell GT System gel running system	Bio-Rad	N/A
Chemidoc MP Imaging System	Bio-Rad	N/A
New Brunswick Innova 40 benchtop shaker	Innova	N/A
Eppendorf ThermoMixer C	Eppendorf	N/A
ProteinSimple Wes System	ProteinSimple (Bio-Techne)	N/A