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. 2022 Oct 26;3(4):101785. doi: 10.1016/j.xpro.2022.101785

Plasmid linearization by restriction enzyme digestion

Reagent Amount
Plasmid (Concentration 1 μg/μL) 4 μL
3.1 NEBuffer™ 5 μL
ddH2O (To 50 μL) 37 μL
BamHI enzyme 4 μL