Official websites use .gov
A
.gov website belongs to an official
government organization in the United States.
Secure .gov websites use HTTPS
A lock (
) or https:// means you've safely
connected to the .gov website. Share sensitive
information only on official, secure websites.
PCR reaction master mix
| Reagent | Amount |
|---|---|
| Template incubated with betaine (DNA + betaine + water) | 15 μL |
| 5 M Betaine | Add 3.5 μL (Final 5 μL) |
| ddH2O (To 50 μL) | Add 15 μL (Final 25.5 μL) |
| Phusion™ G-C Buffer | 10 μL |
| 100 μM Reverse primer | 2.5 μL |
| 100 μM Forward primer | 2.5 μL |
| 10 mM dNTP | 1 μL |
| Phusion™ High-Fidelity DNA Polymerase (2 U/μL) | 0.5 μL |
